Calculating the Secrets of Life Contributions of the Mathematical Sciences to Molecular Biology (1995) / Chapter Skim
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Pages 42-46
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From page 42... ...
Once a gene has been determiner! to lie between two genetic markers, the geneticist must produce a physical map-consisting of overlapping clones spanning the chromosomal region between the two flanking markers.
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possible fingerprints include the following: Complete DNA sequence. For very small genomes such as those of viruses, it is practical to reassemble the genome from very short subclones of length ~300 to 500 base pairs.
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To make a restriction map, one can use the method of partial digestion: one radioactively labels one end of a clone, adds a restriction enzyme briefly so that only a random selection of the sites are cut, and measures the lengths of the resulting fragments (Figure 2.~. Restnction maps can be eff~ciently cons~cted for clones of moderate size (up to about 50,000 base pairs)
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constructed a complete physical map of the bacterium Escherichia cold with a genome of 4.6 million base pairs using phage clones containing fragments of about 15,000 base pairs. Restriction fragment sizes.
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construct a physical map of the yeast Saccharomyces cerevisiae with a genome of 13 million base pairs. Content of sequence tagged sites.
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